In the conventional PCR system, it was designed for the detection of the amplified DNA product (amplicon) in an end-point analysis while in the modern real-time PCR machine the measurement of the amplification product accumulation is carried out through reaction progression happening in real-time. It also quantifies the product after each cycle. The actual advantage real-time has over conventional is the determination of template DNA copies with precision. It can be carried out qualitatively and quantitatively. qPCR is the quantitative PCR machine that calculates the number of an initial numb